APO-E

DuplicαRealTime ApoE Genotyping Kit is an in vitro diagnostic test for the qualitative detection, by Real-Time PCR of the following alleles of the ApoE human gene: ApoE*E2 (Cys112-Cys158), ApoE*E3 (Cys112-Arg158) and ApoE*E4 (Arg112-Arg158)

Overview

The apolipoprotein E (ApoE) is a 299 aminoacid glycoprotein synthesized in the liver and nervous system, in surrenal gland, gonads, kidney, musculus striatum and spleen. The three possible alleles of ApoE (E2,E3, E4) encode for the E2, E3 ed E4 isoforms respectively.

In the brain Apo E plays a crucial role in the development and repairment of neuronal structures. Moreover, Apo E is involved in the pathogenesis of the either late onset, familiar or sporadic Alzheimer’s Disease.

Targets:

ApoE*E2 (Cys112-Cys158), ApoE*E3 (Cys112-Arg158) and ApoE*E4 (Arg112-Arg158)
Internal Control (IC)

Diagnostic samples:

Peripheral whole blood samples collected in EDTA

DNA Extraction:

Compatible with the most common DNA Extraction and Purification Systems

Real-Time PCR instruments:

Compatible with the most common Real-Time PCR Systems