Chromosome Y Microdeletion

Chromosome Y Microdeletion is a PCR-based molecular kit to detect by gel electroforesis the Y Chromosome microdeletions associated to male infertility that are located into regions AZFa, AZFb and AZFc

Overview

Infertility is a major health problem affecting 10-15% of couples seeking to have children and a male factor can be identified in about half of these cases. A significant proportion of infertile males are affected either by oligozoospermia or azoospermia.

The recent growth of assisted reproduction techniques has led to the identification of Y chromosome microdeletions into AZF regions correlated to non-obstructive azoospermia, Men with deletions in AZFa and AZFb regions show severe defects in spermatogenesis, whereas deletions of AZFc region can be compatible with residual spermatogenesis.

These Clonit tests are based on Gel Electrophoresis and Reverse Hybridization techniques and it has been developed in agreement with guidelines of European Molecular Genetics Quality Network (EMQN) 2004.

Targets:

sY86 (AZFa), sY127 (AZFb), sY254 (AZFc) regions
sY84 (AZFa), sY134 (AZFb), sY255 (AZFc)
Internal Control (IC)

Diagnostic samples:

Blood samples in EDTA

DNA Extraction:

Compatible with the most common DNA Extraction and Purification Systems

PCR instruments:

Compatible with the most common PCR Systems