Lactose Intolerance (LCT -13910C/T and LCT -22018G/ A)

The lactose intolerance LCT Kit is a qualitative test that allows the allelic discrimination, by Real-Time PCR, of C13910T and G22018A polymorphisms associated to lactose intolerance.

The procedure allows the amplification of the two allelic variants for the polymorphism discriminating the three possible genotypes: Homozygote Wild-Type, Homozygote Mutated and Heterozygote Mutated for both polymorphisms.

Overview:

Many adults have a genetically caused deficiency of the enzyme lactase (LCT gene) which results in intestinal disorders on consumption of milk or milk products. The two main polymorphisms associated with lactose intolerance are LCT -13910C/T and LCT -22018G/ A, located in the regulatory region of the lactase gene. According to current knowledge, homozygous carriers of the wild type variants -13910C/C and -22018G/G develop lactose intolerance, while heterozygous carriers of the polymorphisms -13910C/T and -22018G/A only show corresponding symptoms in stress situations or with intestinal infections.

Target:

C13910T polymorphism
G22018A polymorphism
Internal Control (IC)

Diagnostic samples:

Whole Blood EDTA

Real-Time PCR instruments:

Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
StepOnePlus™ Real-Time PCR System (ThermoFisher SCIENTIFIC)
Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
Versant kPCR AD (Siemens)
CFX96 Real-Time PCR Detection System (Bio-Rad)

DNA Extraction:

Manual Extraction with:

QIAmp DNA mini kit (QIAGEN)

Automatic Extraction with:

EZ1 Advanced XL (QIAGEN)