HLA-B*27
Code
RT-53
CE MarkRT-53
Tests

48 Test

HLA-B*27

The HLA-B*27 kit is an in vitro diagnostic test for determination of the presence of HLA-B*27 allele, by Real-Time PCR. 

 

Ankylosing spondylitis (AS), a chronic inflammatory disease, is strongly associated with the HLA-B27 antigen. This association was first described in 1973 and is the largest genetic factor contributing to the disease.

The identification of the HLA-B*27 alleles is relevant in the clinical follow-up, diagnosis, and treatment of AS.

Target:

  • HLA-B*27 allele
  • Internal Control (IC)

Diagnostic samples:

  • Whole Blood EDTA

Real-Time PCR instruments:

  • Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
  • StepOnePlus™ Real-Time PCR System (ThermoFisher SCIENTIFIC)
  • Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
  • LightCycler® 480 Real-Time PCR System (Roche)
  • Versant kPCR AD (Siemens) 
  • CFX96 Real-Time PCR Detection System (Bio-Rad)

DNA Extraction:

Manual Extraction with:

  • QIAmp DNA mini kit  (QIAGEN)

Automatic Extraction with:

  • EZ1 Advanced XL (QIAGEN)