DUPLICA RealTime HLA-B*5701 EZ Type Kit

DuplicαRealTime HLA-B*5701 EZ Type Kit is an in vitro diagnostic test for the detection of the presence of the HLA-B*57:01 allele in genomic DNA, by Real-Time PCR.

Overview

The HLAB* 57 family consists of at least 57 closely related genes. Around 5% to 8% of the Western European and Hispanic populations express 57-like antigens, so this represents a common HLA genotype. This genotype is less frequent in other ethnic groups, and is found in <2% of people of African origin.

Abacavir (ABC) is a nucleoside reverse-transcriptase inhibitor antiretroviral indicated in combination with other antiretroviral agents for the treatment of HIV-1 infection, in adult and paediatric patients. Abacavir is associated with potentially serious hypersensitivity reactions in 3–9% of Caucasian patients that require immediate and permanent discontinuation of the drug.

It has been shown that carriers of the allele 57:01 of the HLA locus B (HLA-B*57:01) are at high risk for developing Abacavir Hypersensitivity Reaction (ABC-HSR) and that prospective genetic screening for HLA-B*57:01 greatly diminishes or abolishes the occurrence of ABC-HSR. In July 2008, the US FDA approved a label change for Abacavir to include a boxed warning indicating that patients who carry the HLA-B*57:01 allele are at high risk for experiencing a hypersensitivity reaction to Abacavir and a recommendation to screen for HLA-B*57:01 prior to the initiation of Abacavir therapy.

Targets:

HLA-B*57:01 allele
Internal Control (IC)

Diagnostic samples:

Peripheral whole blood samples collected in EDTA

DNA Extraction:

Compatible with the most common DNA Extraction and Purification Systems

Real-Time PCR instruments:

Compatible with the most common Real-Time PCR Systems