quanty Chikungunya
Code
RT-05
CE Mark
Tests
48 test
quanty Chikungunya
The quanty Chikungunya system is a quantitative test that allows the RNA amplification in Real-Time PCR, of nsP2 region of Chikungunya (CHIK).
The Procedure allows the detection and quantification of the RNA target by means a One step retro-amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyzer (thermal cycler integrated with a system for fluorescence detection and a dedicated software).
Target:
- nsP2 region of Chikungunya
- Internal Control (IC)
Standard for quantification:
- synthetic RNA corresponding to nsP2 region 100.000 cps/µL
- synthetic RNA corresponding to nsP2 region 10.000 cps/µL
- synthetic RNA corresponding to nsP2 region 1.000 cps/µL
- synthetic RNA corresponding to nsP2 region 100 cps/µL
Diagnostic samples:
- Serum
- Plasma
Real-Time PCR instruments:
- Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
- Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
RNA Extraction:
Manual Extraction with:
- QIAmp Viral RNA Mini Kit (QIAGEN)
Automatic Extraction with:
- EZ1 Advanced XL (QIAGEN)
- QIAsymphony SP (QIAGEN)