quanty Measles

The quanty Measles system is an in vitro diagnostic test for identification and quantification of the N3 region of Measles virus genome, by one-step RT- Real-Time PCR.

Measles is one of the most contagious of all viral infectious diseases and is caused by a virus in the paramyxovirus family. It remains an important cause of death among young children globally, despite the availability of a safe and effective vaccine.

In the decade before the live measles vaccine was licensed in 1963 high number of deaths were reported annually. However measles cases and outbreaks still occur every year because measles is still commonly transmitted in many parts of the world, including countries in Europe, the Middle East, Asia, the Americas, and Africa. The COVID-19 pandemic, which started in late 2019, led to an interruption of routine vaccination services in many countries and cancellation or postponement of planned SIAs. As a result, 29 countries had postponed measles campaigns as of June 2020,7 with 18 of them experiencing ongoing measles outbreak.

Having measles, mumps, or rubella during pregnancy might increase the chance of stillbirth (fetal death after 20 weeks). A measles or mumps infection near the time of delivery might also increase the chance of a baby being born with the infection, although this is thought to be rare.

Target:

N3 region of Measles virus genome
Internal Control (IC)

Standard for quantification:

Synthetic RNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)

Diagnostic samples:

Serum
Urine

Real-Time PCR instruments:

Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
LightCycler® 480 Real-Time PCR System (Roche)

RNA Extraction:

Manual Extraction with:

QIAmp Viral RNA mini kit (QIAGEN)

Automatic Extraction with:

EZ1 Advanced XL (QIAGEN)