Measles/Rubella

The Measles/Rubella system is a molecular assay for identification of Measles and Rubella virus genome, by one-step RT- Real-Time PCR. 

Measles is a highly infectious virus and globally one of the leading causes of childhood mortality. Initial symptoms usually occur 10–12 days after infection and comprise high fever, runny nose, bloodshot eyes and tiny white spots on the inside of the mouth. Several days later, a rash develops on the face and neck, and will gradually spread downwards. The most severe complications of measles include blindness, encephalitis (an infection that causes brain swelling), severe diarrhea, and pneumonia, among others. 

Rubella is usually mild when experienced in childhood and adolescence, but it can often lead to serious and even fatal complications for the foetus when an unprotected woman is infected in her early pregnancy. An infection during pregnancy can result in miscarriage or congenital defects in the unborn child.

The two illnesses share some signs and symptoms, such as the red rash why it is important to differentiate them. Anyway, fortunately the measles-mumps-rubella (MMR) vaccine is safe, highly effective and provides lifelong protection against these diseases.

Targets:

• N3 gene of Measles Virus 
• E1 region of Rubella virus genome
• Internal Control (IC)

Diagnostic samples:

• Different clinical speciemens

Real-Time PCR instruments:

• Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
• Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)

RNA Extraction:

Manual Extraction with:

• QIAmp Viral RNA mini kit  (QIAGEN)

Automatic Extraction with:

• EZ1 Advanced XL (QIAGEN)

• QIAsymphony SP (QIAGEN)