Flu A - Flu B - Sars CoV-2

Flu A - Flu B - Sars CoV-2 is an in vitro diagnostic test for the simultaneous detection e differentiation of SARS-CoV-2 (N gene), Flu A (M1 gene) and Flu B (NS2), by one-step Real-Time RT-PCR.

Overview

Severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) is a strain of coronavirus that causes COVID-19 (coronavirus disease 2019), the respiratory illness responsible for the ongoing COVID-19 pandemic. Patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A and influenza B show in most cases comparable or very similar manifestations but the therapeutic approaches of these respiratory viral infections are different and require an accurate diagnosis. The Flu A - Flu B - Sars CoV-2 kit allows simultaneous detection and differentiation of SARS-CoV-2, influenza A, influenza B in respiratory tract samples representing a reliable and important diagnostic aid especially during the cold season.

Targets:

N regionof SARS-CoV-2 genome
M1 gene of Influenza A genome
Internal Control (IC)

Diagnostic samples:

nasopharyngeal/oropharyngeal swabs

Real-Time PCR instruments:

Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
CFX96 Real-Time PCR Detection System (Bio-Rad)

RNA Extraction:

Manual Extraction with:

QIAmp Viral RNA Mini Kit (QIAGEN)

Automatic Extraction with:

CloNext 12 or CloNext 24 (ref. OP01018/OP01019) robotic workstation
Duplicα®Prep (ref. EDI001 or EDI003) robotic workstation
QIAsymphony SP/AS (Ref. 9001297/9001301, QIAGEN)
Automated Liquid Handlers equipped with the protocol for the Mag-Bind® Viral RNA XPress Kit from Omega BIO-TEK