48 Tests
Available for QiaSymphony SP/AP - Ref. QS-13
quanty EBV
The quanty EBV system is an in vitro diagnostic test for identification and quantification of Epstein Barr virus (EBV) genome, by Real-Time PCR.
Epstein-Barr virus (EBV) is the causative agent of infectious mononucleosis (IM). It was first discovered in cells isolated from African Burkitt’s lymphoma. EBV is highly prevalent since it affects more than 90% of individuals worldwide. As other herpesviruses, following a primary infection, EBV has a latency phase where it infects epithelial cells, enters the circulating B lymphocyte, and persists for the life in a latent state. Typically, the primary infection is asymptomatic and occurs during childhood. However, the infection could lead to IM if it occurs in adults. In addition, this virus has been linked to a wide range of malignancies, such post-transplant lymphoproliferative diseases (PTLDs), nasopharyngeal carcinoma (NPC), Hodgkin’s lymphoma, and gastric carcinoma. Epstein-Barr virus detection and quantification by Real-Time PCR is essential to quickly set up an appropriate antiviral treatment and prevent serious complications.
Target:
- BamHI-W region of EBV genome
- Internal Control (IC)
Standard for quantification:
- Synthetic DNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)
Diagnostic samples:
- Blood EDTA
- Plasma EDTA
- Liquor (CSF)
Real-Time PCR instruments:
- Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
- Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
- CFX96 Real-Time PCR Detection System (Bio-Rad)
DNA Extraction:
Manual Extraction with:
- QIAmp DNA mini kit (QIAGEN)
Automatic Extraction with:
- EZ1 Advanced XL (QIAGEN)
- QIAsymphony SP (QIAGEN)