quanty HHV-6

The quanty HHV 6 system is an in vitro diagnostic test for identification and quantification of U38 gene region of Human herpesvirus 6 genome, by Real-Time PCR.

Human herpesvirus (HHV)--6 infections are ubiquitous. Primary infection with the virus is associated with roseola infantum (exanthem subitum) but infection or reactivation under immunocompromised conditions, such as bone marrow or solid organ transplantation, can often result in serious clinical manifestations. HHV 6 detection and quantification by Real-Time PCR is essential to quickly set up an appropriate treatment and often prevent serious complications.

Target:

U38 gene region of HHV 6 genome
Internal Control (IC)

Standard for quantification:

Synthetic DNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)

Diagnostic samples:

Whole Blood
Liquor (CSF)

Real-Time PCR instruments:

Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
StepOnePlus Real-Time PCR (ThermoFisher SCIENTIFIC)
Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
CFX96 Real-Time PCR Detection System (Bio-Rad)
Versant kPCR AD (Siemens)

DNA Extraction:

Manual Extraction with:

QIAmp DNA mini kit (QIAGEN)

Automatic Extraction with:

EZ1 Advanced XL (QIAGEN)
QIAsymphony SP (QIAGEN)