quanty BK

The quanty BK virus system is an in vitro diagnostic test for identification and quantification of the VP-1 region of BK virus genome by Real-Time PCR.

BK virus associated diseases (nephropathy, ureteral stenosis, hemorrhagic cystitis) are detected in immunocompromised patients who had renal transplantation or hematopoietic stem cell transplantation, although BKV can occur also in a low percentage of asymptomatic immunocompetent individuals. BK virus detection and quantification by Real-Time PCR is essential to quickly set up an appropriate antiviral treatment and prevent serious complications.

Target:

VP-1 region of BK virus genome
Internal Control (IC)

Standard for quantification:

Synthetic DNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)

Diagnostic samples:

Blood EDTA
Plasma
CSF
Urine

Real-Time PCR instruments:

Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
LightCycler® 480 Real-Time PCR System (Roche)
Versant kPCR AD (Siemens)
CFX96 Real-Time PCR Detection System (Bio-Rad)
mic qPCR cycler (Bio Molecular System)

DNA Extraction:

Manual Extraction with:

QIAmp DNA mini kit (QIAGEN)

Automatic Extraction with:

EZ1 Advanced XL (QIAGEN)
QIAsymphony SP (QIAGEN)