quanty Enterovirus

The quanty Enterovirus system is an in vitro diagnostic test for identification and quantification of of 5’UTR region of Enterovirus RNA, by RT-Real-Time PCR.

There are more than 100 subtypes of enteroviruses that infect humans, including the well-known enterovirus 71 (EV71), enterovirus D68 (EVD68), coxsackieviruses A and B, and poliovirus (PV), and several subtypes of these induce hand-foot-and-mouth disease (HFMD) epidemics every year. Poliovirus classically causes a paralytic poliomyelitis with weakness and paralysis. Other enteroviral diseases include upper respiratory infections, herpangina, myositis, myocarditis, aseptic meningitis, encephalitis. Patients with B-cell immune defects are susceptible to chronic enterovirus infections including chronic meningoencephalitis. Patients undergoing haematopoietic stem cell transplantation (HSCT) may develop potentially life-threatening gastroenteritis and viral pneumonitis. Molecular diagnostics and characterisation of circulating enterovirus strains are the first step towards effective and continuous surveillance activities, which will be used to provide better estimation on EV disease burden.

Target:

5'UTR Region
Internal Control (IC)

Standard for quantification:

Synthetic RNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)

Diagnostic samples:

Plasma
Liquor (CSF)

Real-Time PCR instruments:

Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
CFX96 Real-Time PCR Detection System (Bio-Rad)
LightCycler® 480 Real-Time PCR System (Roche)
Versant kPCR AD (Siemens)

RNA Extraction:

Manual Extraction with:

QIAmp Viral RNA mini kit (QIAGEN)

Automatic Extraction with:

EZ1 Advanced XL (QIAGEN)
QIAsymphony SP (QIAGEN)